The Association of Midwest Fish and Game Law Enforcement Officers (AMFGLEO) is a 501(c)(3) organization and was chartered February, 1944 in Lincoln, Nebraska. There are currently 23 member agencies from Canada and the United States. The AMFGLEO meets every year taking turns in different provinces and states. The meetings have been beneficial to the member agencies. Over the years, the AMFGLEO has become the lead group among wildlife enforcement organizations in the development and maintenance of training for the field officers that protects the natural resources and benefits the citizens of our states, provinces, and countries.

The AMFGLEO annual meeting is in the spring/summer. The sessions are designed to allow field personnel and law enforcement staff to learn about new issues in wildlife law enforcement, discuss items of mutual interest and develop and maintain contacts to facilitate interstate and international investigations of natural resource violations. The AMFGLEO has standing committees that survey member agencies and present reports on topics such as training issues, legislation and forensics. The AMFGLEO also recognizes the Officer of the Year of any member agency.

The annual meeting is on a three-year rotation cycle. One year is a stand-alone meeting with AMFGLEO members. The next year it is a combined meeting with the Midwest Association of Fish and Wildlife Agencies (MAFWA). The third year is a combined meeting with the Midwest Investigators to complete the three-year rotation cycle

The AMFGLEO developed the Wildlife Forensic Field Manual. The printed 5th Edition was published in December, 2022 and is now available in 2024 as a digital download. In addition, AMFGLEO updated the printed Time of Death in Selected Wildlife Species manual, first published by Nebraska Game & Parks, and was published by AMFGLEO in early 2023 and is now available in 2024 as a digital download.

In addition, the AMFGLEO has supported wildlife enforcement research through grants. Monetary support has been provided to the North American Wildlife Enforcement Officers Museum and Educational Center that is located at the Peace Gardens on the US/Canada border between North Dakota and Manitoba. All proceeds generated through this organization are used to support conservation law enforcement in all member provinces and states.

Updated 1/24/2024